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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Mol Biol. 2017 Jun 30;429(16):2542–2555. doi: 10.1016/j.jmb.2017.06.018

Fig. 3.

Fig. 3.

Delineating the HIV-1 sequence required to package a heterologous RNA into particles. (a) General structures of an HIV-1 helper and vector. Plasmid SG-NoSL expresses codon-optimized Gag-CeFP fusion protein whereas HIV-1 vector 1-AAG contains a mutation that abolished the gag translation start codon from AUG to AAG. (b) General structures of a firefly luciferase reporter constructs and the inserted HIV-1 sequences. The length of the gag sequence is indicated in the names of the constructs. (c) Packaging of heterologous RNAs. (d) Cellular expression of the reporter RNAs monitored by firefly luciferase activities. Firefly luciferase activities were normalized to that from No-HIV-luc. Results from three independent experiments are summarized; error bars indicate the standard deviations.