Figure 1.

Effect of silencing of sirtuin family members on cancer cell viability under diverse types of cellular stress. SRB assay was used to assess cell viability of (A) U2OS, (B) Mero-14, and (C) REN cells in which SIRT1 (7–12), SIRT3 (13–18), or SIRT5 (19–24) had been transfected to silence the respective sirtuin family member as indicated. Cells were treated with rotenone, etoposide, and resveratrol for 48 h with the indicated concentrations. Bars 1–6 represent cell viability of cells transfected with siRNA scramble. Error bars represent standard error of means of three or more independent experiments. One-way analysis of variance (ANOVA) and Tukey's multiple comparisons post-test were used for the analysis of the data. p < 0.05 was considered to be statistically significant. p < 0.001 is indicated with (*), p < 0.01 with (#), and p < 0.05 with ($).