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. 2019 Sep 24;9:949. doi: 10.3389/fonc.2019.00949

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Copyright © 2019 Garva, Thepmalee, Yasamut, Sudsaward, Guazzelli, Rajendran, Tongmuang, Khunchai, Meysami, Limjindaporn, Yenchitsomanus, Mutti, Krstic-Demonacos and Demonacos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of silencing of SIRT5 on SIRT1 and on SIRT3 protein levels. Protein levels of SIRT1, SIRT3, and SIRT5 were determined by SDS PAGE followed by Western blot. Silencing of SIRT5 gene expression in (A) U2OS, (B) Mero-14, and (C) REN cells. Protein levels were analyzed using antibodies specific for the SIRT1, SIRT3, or SIRT5. Actin was used as a loading control. Representative Western blot is shown. Image J was used for quantification. Values are normalized to actin and then to control. Error bars represent standard error of the mean of three independent experiments. One-way analysis of variance (ANOVA) and Tukey's multiple comparisons post-test were used for the analysis of data. p < 0.05 was considered to be statistically significant. p < 0.001 is indicated with (***), p < 0.01 with (**), and p < 0.05 with (*).