Fig 3.

A) Nuclei are more compressed as MCAs grow in size. Nuclear volume as a function of MCA volume in spherical MCAs. The number of nuclei range from 20 to 90 in each MCA; Plotted values are mean volumes, with error bars depicting standard deviation. Each data point represents the total data from 2 to 4 MCAs. As the MCAs grow in size, the average nuclear size decreases with a commensurate increase in average pressure. B) Nuclei in elliptical MCAs are less compressed along the MCA major axis, as compared to the minor axis, or that in spherical MCAs. changes in elliptical multicellular aggregate from the center towards the edges of the MCAs. This figure presents the volume of the nuclei as a function of position in the MCA; volume of the nuclei in the: spherical (red stars) MCAs, elliptical MCAs from the center along the major axis (blue circles), elliptical MCAs from the center along the minor axis (black squares). Data are taken from one elliptical and one spherical (both 2100+/-100 x 10³ μm³ volume) MCA, and represent mean and standard deviation.