Figure 2.
Sodium chloride-esculin spot test. Filter paper impregnated with sodium chloride-esculin solution and placed on a standard microscope slide. Top slide: Negative control used is Staphylococcus aureus. Positive control used are Enterococcus faecalis ATCC® 29212™ and a known Enterococcus faecalis (VITEK 2 systems identified). 2nd and 3rd slide: Positive reactions are indicated by the inoculation spot turning black 30–60 minutes later