Figure 2.

Impact of the mitochondrial Ca²⁺ uniporter (MCU)-silencing on cytosolic Ca²⁺ oscillations. (A) Cytosolic Ca²⁺-probe fluorescence in HeLa cells stimulated with 3 µm histamine. The Ca²⁺ oscillations induced by histamine are drastically different in control cells (blue) compared with those in MCU-silenced cells (red), thus showing how Ca²⁺ handling by mitochondria can affect cytosolic Ca²⁺ signals. (B) Mean period of the Ca²⁺ oscillations shown in panel A. In MCU-silenced cells, the period of the cytosolic Ca²⁺ oscillations is much increased, as compared with control cells. (C) Coefficient of variation (CV) (standard deviation/mean) of the time interval between two successive peaks of these oscillations. The MCU-silenced cells display a lower CV, which is associated with more regular spikes. (The data are taken, with permission, from Wacquier et al. 2016.)