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. 2019 Oct 1;33(19-20):1428–1440. doi: 10.1101/gad.328773.119

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© 2019 Lee et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — The level of EZH2 automethylation is significantly reduced in H3K27M containing cells. (A) EZH2 automethylation is significantly reduced in 293T-Rex cells containing doxycycline inducible H3.3K27M or H3.3K27WT. Western blot analyses of EZH2-K514me3, EZH2, GAPDH, H3K27me2, H3K27me3, and total histone H3 levels. Asterisks indicate nonspecific bands. (B) EZH2 automethylation is significantly reduced in diffuse intrinsic pontine gliomas (DIPGs) containing the histone H3K27M mutation. Western blot analysis of EZH2-K514me3, EZH2, GAPDH, H3K27me2, H3K27me3, and total histone H3 levels in the following glioma cell lines: H3K27M glioma (DIPG IV and DIPG XIII) and H3WT glioma (pcGBM2 and DIPG X). Asterisks indicate nonspecific bands.