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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Sci Immunol. 2018 Sep 7;3(27):eaaq0491. doi: 10.1126/sciimmunol.aaq0491

Fig. 2 – Existing applications of immune cell imaging in various tissues.

Fig. 2 –

(A) A wide range of mouse tissue sites is adapted for single cell imaging. (B) Intravital micrograph showcasing detection of two immune cell types, namely CD4+ T cells (red) and CX3CR1+ macrophages (green) in pancreatic islets. β-cells (blue) were visualized with exendin-4–like neopeptide conjugated to the fluorescent dye Se-Tau-647 and the vasculature was detected with 500 K MW dextran conjugated to a Pacific blue dye (grey). (C-D) Intravital micrographs showcasing detection of cytokines produced by immune cells in live mice, here in tumor tissues. Panel C shows IFN-γ-producing lymphocytes (magenta) and panel D shows IL-12b-producing myeloid cells (cyan). Tumor cells are also shown (grey). All scale bars represent 10 μm. Credit: A. Kitterman/AAAS