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. 2019 May 28;186(5):754–766. doi: 10.1111/bjh.15970

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© 2019 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Distribution of memory T‐cell subsets in ATIR101 cells before and after photodepletion. Memory T‐cells were analysed pre‐ and post‐photodepletion (PD) by flow cytometry for CD62L, CD45RA and CD45RO expression and gated on CD4 (A–D) or CD8 (E–H) cells. CD45RO ⁻ CD45RA ⁺ CD62L⁺ defined T_NAIVE; CD45RO ⁺ CD45RA ⁻ CD62L⁻, effector memory (T_EM) cells; CD45RO ⁺ CD45RA ⁻ CD62L⁺, central memory (T_CM); and CD45RO ⁻ CD45RA ⁺ CD62L⁻, terminally differentiated effector (T_EMRA) cells. Bars represent mean values (n = 7) and P values correspond to paired t‐tests between pre‐ and post‐photodepletion results.