Table 1.

Experimental plan for the in vivo characterisation of the patch and myocardial tissue

Large Animals White × Landrance Pigs (n = 4)			Patch characterisation after explant	Cardiac tissue characterisation
	Follow‐up	Treatment		Biopsies for biochemical analysis (ischaemic and remote area)	Heart sections Histological analysis	Myocardial sample FTIR, HPLC analysis
1_AMI	24 hr	No patch		10′ before LAD, 45′ after LAD, 10′ LAD reopening, 60′ LAD reopening	Ischaemic area Remote area
2_AMI + MMP	24 hr	Patch without adenosine	HPLC, FTIR, SEM	10′ before LAD, 45′ after LAD, 10′ LAD reopening, 60′ LAD reopening	Ischaemic area Remote area	Ischaemic area (proximal and distal sections) Remote area
3_AMI + ADMMP	24 hr	Patch with adenosine	HPLC, FTIR, SEM	10′ before LAD, 45′ after LAD, 10′ LAD reopening, 60′ LAD reopening	Ischaemic area Remote area	Ischaemic area (proximal and distal sections) Remote area
4_CMI + ADMMP	3 months	Patch with adenosine		10′ before LAD, 45′ after LAD, 10′ LAD reopening, 60′ LAD reopening	Ischaemic area Remote area	Ischaemic area (proximal and distal sections) Remote area

Note. 1_AMI: animal subjected to LAD to induce ischaemia, follow up at 24‐hr acute myocardial infarct model; MMP: multilayer microstructured patch (patch nonloaded with adenosine); ADMMP: adenosine delivery multilayer microstructured patch (patch loaded with adenosine); 2_AMI + MMP: animal subjected to LAD to induce ischaemia, follow up at 24 hr acute myocardial infarct model treated with patch nonloaded with adenosine (MMP); 3_AMI + ADMMP: animal subjected to LAD to induce ischaemia, follow up at 24 hr acute myocardial infarct model treated with patch loaded with adenosine (ADMMP); 4_CMI + ADMMP: animal subjected to LAD to induce ischaemia, follow up at 3 months chronic myocardial infarct model treated with patch loaded with adenosine (ADMMP).