Figure 6.

Effect of gefitinib on expression of IL‐12, IFN‐γ, TNF‐α, and NOS2 in the eye and brain as well as on the induction of systemic cellular and humoral immunity. (a) B6 mice were infected with ME49 Toxoplasma gondii, treated for 14 days with gefitinib or vehicle beginning on day 6 postinfection. Levels of IL‐12 p40, IFN‐γ, TNF‐α, and NOS2 mRNA in the eyes and brains were examined using qPCR. Each group contained nine to 10 mice pooled from two experiments. Results are shown as the mean + SEM. (b) Serum levels of IFN‐γ and TNF‐α at day 14 postinfection. (c, d) Splenocytes collected at day 14 postinfection were incubated with or without T. gondii lysate antigens (TLA), and supernatants were collected to measure IL‐12 p40, IFN‐γ, and TNF‐α (c) or measure nitric oxide by Griess reaction (d). Bars are mean + SEM of nine to 10 samples per group. (e) Spleen and lung lysates were subjected to immunoblot using Ab to IRGM3 and actin. (f) Serum titres of anti‐T. gondii IgG were measured by ELISA. Bars are mean + SEM of nine to 10 samples per group