Figure 8.

Gefitinib fails to control ocular and cerebral toxoplasmosis in Beclin 1‐deficient mice. Becn1 ^+/+ and Becn1 ^+/− mice were infected with T. gondii tissue cysts i.p., and at 2 weeks postinfection, they were treated with gefitinib or vehicle for 2 weeks. The eyes and brains were collected 4 weeks postinfection. (a) Toxoplasma gondii B1 gene was examined in the eye by using qPCR. Bar graph represents mean + SEM of nine mice pooled from two experiments. Image from gefitinib‐treated Becn1 ^+/+ mouse shows better preservation in retinal architecture. Gefitinib does not improve retinitis in Becn1 ^+/− mice. Retina from gefitinib‐treated mouse shows invasion by retinal pigment epithelial cells (arrowhead). H&E; 200×. Bar, 50 μm. Histopathology scores are shown in a bar graph as mean + SEM of nine samples per group pooled from two experiments. (b) Numbers of T. gondii tissue cysts per brain. Gefitinib does not improve encephalitis in Becn1 ^+/− mice. Periodic acid Schiff haematoxylin original magnification 200×. Bar, 50 μm. Bar graphs represent mean + SEM of nine mice pooled from two experiments. **P < .01; n.s., not significant