Table 3.

Effect of P, and Ca (PCa), and phytase (Phy) supplementation on concentrations of InsP isomers and myo-inositol (μmol/g DM) in the crop and in proventriculus and gizzard of gnotobiotic broiler chickens fed the experimental diets from day 10 to day 15¹.

	Dietary treatment2					P-values
	PCa−		PCa+
	Phy−	Phy+	Phy−	Phy+	Pooled SEM	PCa	Phy	PCa × Phy
Crop
InsP6	15.0	13.9	14.9	12.9	0.31	0.059	<0.001	0.097
Ins(1,2,4,5,6)P5	0.9	0.6	0.9	0.5	0.03	0.427	<0.001	0.131
Ins(1,2,3,4,5)P5	0.5	0.5	0.6	0.6	0.02	0.001	0.293	0.293
Ins(1,2,3,4,6)P5	0.2	<LOQ3	0.2	n.d.4	0.04	0.391	–	–
Ins(1,2,5,6)P4	n.d.	1.0	n.d.	2.5	0.25	0.001	–	–
InsP3x5	n.d.	0.2	n.d.	0.5	0.03	0.003	–	–
Myo-inositol	1.0	1.0	0.8	0.9	0.03	0.003	0.165	0.474
Proventriculus+Gizzard
InsP6	9.0	2.7	8.9	1.8	0.19	0.022	<0.001	0.057
Ins(1,2,4,5,6)P5	0.4	0.3	0.5	0.2	0.04	0.169	<0.001	0.063
Ins(1,2,3,4,5)P5	0.3	0.8	0.3	0.5	0.07	0.066	<0.001	0.066
Ins(1,2,5,6)P4	n.d.	3.2	n.d.	3.3	0.21	0.528	–	–
InsP3x5	n.d.	0.7	n.d.	0.9	0.30	0.161	–	–
Myo-inositol	0.6b	1.0a	0.4c	0.6b	0.05	<0.001	<0.001	0.023

¹Data are given as treatment means; n = 4 pens.

²Calculated composition: PCa−, 4.1 g P/kg DM and 6.2 g Ca/kg DM; PCa+, 6.9 g P/kg DM and 10.4 g Ca/kg DM; Phy−, 0 FTU/kg; Phy+, 1,500 FTU/kg as fed.

³ <LOQ, not quantifiable in the majority of samples.

⁴n.d., not detectable in the majority of samples.

⁵At least one of the following isomers: Ins(1,2,6)P₃, Ins(1,4,5)P₃, Ins(2,4,5)P₃.

^a–cMeans within a row not showing a common superscript differ (P < 0.05).