Table 3.
Analyzed nutrient concentrations in the experimental diets (g/kg DM unless otherwise stated).
| Treatment1 | SB1 | SB2 | SB3 | SB4 | SR1 | SR2 | SR3 | SR4 | SF1 | SF2 | SF3 | SF4 | SB1+ | SB2+ | SB3+ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Monocalcium phosphate Main protein source | Without monocalcium phosphate | With monocalcium phosphate | |||||||||||||
| Soybean meal | Soybean meal + rapeseed meal | Soybean meal + sunflower meal | Soybean meal | ||||||||||||
| Enzyme2 | NES | 1,500 Phy | 3,000 Phy | Prot | NES | 1,500 Phy | 3,000 Phy | Prot | NES | 1,500 Phy | 3,000 Phy | Prot | NES | 1,500 Phy | 3,000 Phy |
| DM (g/kg) | 889 | 889 | 889 | 888 | 893 | 890 | 890 | 892 | 894 | 895 | 893 | 893 | 889 | 888 | 888 |
| P | 4.1 | 4.2 | 4.1 | 4.2 | 5.3 | 5.1 | 5.2 | 5.2 | 5.3 | 5.3 | 5.3 | 5.2 | 5.6 | 5.6 | 5.7 |
| InsP6-P | 2.8 | 2.8 | 2.7 | 2.6 | 3.4 | 3.4 | 3.4 | 3.4 | 3.7 | 3.6 | 3.6 | 3.5 | 2.8 | 2.8 | 2.8 |
| Ca | 9.2 | 8.9 | 9.6 | 9.4 | 9.6 | 9.5 | 9.3 | 9.6 | 10.0 | 10.2 | 10.0 | 9.7 | 10.3 | 10.5 | 10.2 |
| Ca/P ratio | 2.2 | 2.1 | 2.3 | 2.3 | 1.8 | 1.8 | 1.8 | 1.8 | 1.9 | 1.9 | 1.9 | 1.9 | 1.9 | 1.9 | 1.8 |
| Gross energy (MJ/kg DM) | 19.2 | 19.3 | 19.2 | 19.2 | 19.9 | 19.9 | 19.9 | 19.9 | 19.8 | 19.8 | 19.8 | 19.8 | 19.3 | 19.3 | 19.3 |
| CP | 243 | 244 | 242 | 243 | 231 | 230 | 233 | 231 | 233 | 233 | 235 | 233 | 245 | 245 | 243 |
| Amino acids | |||||||||||||||
| Ala | 12.5 | 12.7 | 12.3 | 12.5 | 11.8 | 11.7 | 11.7 | 11.6 | 11.6 | 11.9 | 11.8 | 20.0 | 12.9 | 12.9 | 12.8 |
| Arg | 16.6 | 16.8 | 16.5 | 16.7 | 14.5 | 14.5 | 14.6 | 14.5 | 16.9 | 17.0 | 16.8 | 17.0 | 17.0 | 17.0 | 16.9 |
| Asx3 | 26.6 | 26.8 | 26.4 | 26.7 | 21.9 | 21.9 | 21.8 | 21.7 | 23.8 | 23.8 | 23.6 | 24.1 | 27.6 | 27.7 | 27.5 |
| Cys | 3.9 | 4.0 | 4.0 | 4.1 | 4.4 | 4.3 | 4.3 | 4.2 | 4.0 | 4.0 | 3.9 | 4.0 | 4.0 | 4.0 | 3.9 |
| Glx4 | 45.0 | 45.5 | 44.8 | 45.3 | 41.7 | 41.8 | 41.7 | 41.3 | 44.7 | 44.5 | 44.1 | 45.1 | 46.8 | 46.9 | 46.7 |
| Gly | 10.4 | 10.3 | 10.2 | 10.3 | 10.3 | 10.3 | 10.3 | 10.2 | 11.3 | 11.4 | 11.2 | 11.4 | 10.6 | 10.6 | 10.5 |
| His | 7.2 | 7.1 | 7.1 | 7.3 | 7.3 | 7.1 | 7.0 | 6.9 | 6.9 | 6.9 | 6.9 | 7.1 | 7.8 | 7.8 | 7.7 |
| Ile | 10.4 | 10.5 | 10.1 | 10.1 | 8.3 | 8.7 | 9.0 | 8.8 | 9.4 | 9.5 | 9.4 | 9.2 | 9.9 | 9.9 | 9.8 |
| Leu | 21.1 | 21.2 | 20.8 | 21.1 | 18.7 | 18.9 | 18.9 | 18.7 | 18.9 | 18.9 | 18.8 | 19.0 | 21.5 | 21.5 | 21.4 |
| Lys | 13.5 | 13.4 | 13.3 | 13.4 | 12.0 | 12.0 | 12.1 | 12.0 | 11.1 | 11.1 | 11.0 | 11.2 | 13.7 | 13.7 | 13.6 |
| Met | 3.8 | 3.8 | 3.7 | 3.8 | 3.9 | 4.0 | 4.0 | 3.9 | 4.4 | 4.4 | 4.4 | 4.4 | 3.8 | 3.9 | 3.9 |
| Phe | 12.5 | 12.7 | 12.4 | 12.6 | 10.7 | 10.8 | 10.8 | 10.7 | 11.6 | 11.6 | 11.5 | 11.6 | 12.7 | 12.7 | 12.7 |
| Pro | 14.1 | 14.2 | 14.0 | 14.1 | 14.0 | 13.7 | 13.7 | 13.8 | 12.7 | 12.9 | 12.9 | 13.1 | 14.6 | 14.8 | 15.0 |
| Ser | 13.1 | 13.1 | 13.1 | 13.2 | 12.0 | 11.9 | 11.7 | 11.7 | 12.0 | 11.9 | 11.8 | 12.2 | 13.8 | 13.8 | 13.8 |
| Thr | 9.7 | 9.8 | 9.7 | 9.8 | 9.5 | 9.5 | 9.5 | 9.4 | 9.3 | 9.2 | 9.2 | 9.4 | 10.0 | 10.0 | 10.0 |
| Tyr | 8.5 | 8.6 | 8.5 | 8.5 | 7.5 | 7.5 | 7.6 | 7.4 | 7.5 | 7.5 | 7.4 | 7.5 | 8.6 | 8.6 | 8.6 |
| Val | 11.2 | 11.3 | 10.9 | 10.9 | 9.8 | 10.2 | 10.5 | 10.3 | 10.8 | 10.8 | 10.7 | 10.5 | 10.8 | 10.7 | 10.6 |
| Inositol phosphates5 and myo-inositol (μmol/g DM) | |||||||||||||||
| InsP6 | 15.0 | 14.9 | 14.8 | 14.1 | 18.6 | 18.2 | 18.5 | 18.2 | 20.0 | 19.7 | 19.4 | 19.1 | 15.0 | 14.9 | 15.0 |
| Ins(1,2,4,5,6)P5 | 1.2 | 1.2 | 1.2 | 1.1 | 1.6 | 1.6 | 1.6 | 1.5 | 1.4 | 1.4 | 1.4 | 1.4 | 1.2 | 1.2 | 1.3 |
| Ins(1,2,3,4,5)P5 | 0.5 | 0.5 | 0.5 | 0.4 | 0.9 | 0.9 | 0.9 | 1.0 | 0.9 | 0.9 | 0.9 | 0.8 | 0.6 | 0.5 | 0.5 |
| Ins(1,2,3,4,6)P5 | LOQ | LOQ | LOQ | LOQ | 0.5 | 0.5 | 0.5 | 0.5 | LOQ | LOQ | 0.4 | LOQ | LOQ | LOQ | LOQ |
| Myo-inositol6 | 2.5 | 2.6 | 2.6 | nm | 1.9 | 1.9 | 2.0 | nm | 2.3 | 2.3 | 2.4 | nm | 2.4 | 2.4 | 2.6 |
| Phytase activity (FTU/kg DM) | 60 | 1,480 | 3,620 | 80 | 60 | 1,570 | 3,100 | 80 | 100 | 1,550 | 3,190 | 100 | <60 | 1,850 | 3,600 |
1SB = soybean meal; SR = soybean meal/rapeseed meal; SF = soybean meal/sunflower meal; 1–4 indicate enzyme supplementation in the sequence as described in footnote no. 2; + indicates monocalcium phosphate supplementation.
2NES = no enzyme supplemented; 1,500Phy = 1,500 FTU phytase/kg; 3,000Phy = 3,000 FTU phytase/kg; Prot = 1,600 mg protease/kg.
3Asp and Asn together.
4Glu and Gln together.
5LOQ = below limit of quantification of 0.27 μmol/g DM for Ins(1,2,3,4,6)P5. Concentrations of other measured inositol phosphate isomers were below the respective detection limits in all diets.
6nm = not measured.