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. 2019 Jun 25;98(11):5342–5354. doi: 10.3382/ps/pez354

Table 1.

Statistics of sequencing data for embryonic days 16 and 19 bursal cells from Hy-Line W-36 embryos.

Sample name Raw reads Clean reads Clean bases Error rate (%) Q20 (%) Q30 (%) GC content (%)
ED16–1 110,869,954 108,265,978 16.24G 0.02 95.65 89.81 54.64
ED16–2 93,359,088 91,273,790 13.69G 0.02 95.70 89.83 53.61
ED16–3 104,939,382 98,572,368 14.79G 0.02 94.88 88.45 54.55
ED19–4 97,987,760 93,731,648 14.06G 0.02 96.01 90.45 47.75
ED19–5 84,679,282 81,008,984 12.15G 0.02 95.69 89.70 47.76
ED19–6 78,646,522 75,090,170 11.26G 0.02 95.85 90.13 47.90

ED: embryonic day.

(1) Sample name: the names of samples.

(2) Raw Reads: the original sequencing reads counts.

(3) Clean Reads: number of reads after filtering.

(4) Clean Bases: clean reads number multiply read length, saved in G unit.

(5) Error Rate: average sequencing error rate, which is calculated by Qphred = –10log10€.

(6) Q20: percentages of bases whose correct base recognition rates are greater than 99% in total bases.

(7) Q30: percentages of bases whose correct base recognition rates are greater than 99.9% in total bases.

(8) GC content: percentages of G and C in total bases.