Table 1.
Sample name | Raw reads | Clean reads | Clean bases | Error rate (%) | Q20 (%) | Q30 (%) | GC content (%) |
---|---|---|---|---|---|---|---|
ED16–1 | 110,869,954 | 108,265,978 | 16.24G | 0.02 | 95.65 | 89.81 | 54.64 |
ED16–2 | 93,359,088 | 91,273,790 | 13.69G | 0.02 | 95.70 | 89.83 | 53.61 |
ED16–3 | 104,939,382 | 98,572,368 | 14.79G | 0.02 | 94.88 | 88.45 | 54.55 |
ED19–4 | 97,987,760 | 93,731,648 | 14.06G | 0.02 | 96.01 | 90.45 | 47.75 |
ED19–5 | 84,679,282 | 81,008,984 | 12.15G | 0.02 | 95.69 | 89.70 | 47.76 |
ED19–6 | 78,646,522 | 75,090,170 | 11.26G | 0.02 | 95.85 | 90.13 | 47.90 |
ED: embryonic day.
(1) Sample name: the names of samples.
(2) Raw Reads: the original sequencing reads counts.
(3) Clean Reads: number of reads after filtering.
(4) Clean Bases: clean reads number multiply read length, saved in G unit.
(5) Error Rate: average sequencing error rate, which is calculated by Qphred = –10log10€.
(6) Q20: percentages of bases whose correct base recognition rates are greater than 99% in total bases.
(7) Q30: percentages of bases whose correct base recognition rates are greater than 99.9% in total bases.
(8) GC content: percentages of G and C in total bases.