Figure 2.
Impact of c‐di‐GMP on the ability of A. tumefaciens to kill other bacteria. A. A. tumefaciens strains with either an empty vector control, a plasmid expressing wild‐type SadC or a plasmid expressing a catalytically inactive SadC (psadC*) were incubated for 14 h on I medium with of E. coli DH10B containing pRL662 which harbours a gentamicin cassette. Bacteria were then resuspended in PBS and plated on LB agar containing gentamicin. B. The bacterial strains described above were resuspended in 1/2 Murashige and Skoog (MS) medium (pH 5.5) and immediately injected into the leaves of 6–8‐day‐old N. benthamiana plants. After 14 h incubation, coupons were cut from these leaves, homogenized in PBS and plated on LB supplemented with gentamicin (50 µg ml−1). All experiments are the mean of three independent biological experiments with standard deviation error bars. Statistical significance was determined using students t‐test comparing each strain to the vector control with p < 0.05 *, p < 0.01 **, p < 0.001***.