Figure 3.

Impact of native DGCs on T6SS. A. Expression of a range of six different DGCs whose genes were cloned into wild‐type A. tumefaciens and spotted onto Congo red agar containing 0.5 mM IPTG and Spectinomycin 200 µg ml⁻¹. B. Western blot using Hcp, Tde1, and RpoA (Loading Control) antibodies on supernatants of cells transformed with either empty vector control, pdgcA, patu2091, pdgcC, patu2691, patu1207 or patu5372 from cells grown to early exponential phase in 523 medium. C. Western blot using Hcp, ClpV, TssB, Tde1 and RpoA (Loading Control) antibodies on whole cell lysates of cells transformed with either empty vector control, pdgcA, patu2091, pdgcC, patu2691, patu1207 or patu5372 from cells grown to early exponential phase in 523 medium.