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. 2019 May 29;49(8):1226–1234. doi: 10.1002/eji.201847858

Figure 4.

Figure 4

IFNAR1 signaling is dispensable for Breg induction in vivo. Splenocytes and MACS‐isolated CD19+ B cells from S. mansoni egg‐injected mice (5000 S. mansoni eggs i.p. on day 0 and day 7; section day 14) were restimulated ex vivo with SEA (20 µg/mL) and anti‐CD40 (2 µg/mL) for 2 days. After 2 days of culture, supernatants of isolated B cell and total splenocyte cultures were analyzed for IL‐10 concentration by ELISA (A), and the percentage of IL‐10+ B cells within splenocyte cultures assessed by flow cytometry (B). Data are pooled from two experiments, n = 8–10/group. Data are presented as mean + SEM. Significant differences were determined by one‐way ANOVA followed by Tukey's multiple comparisons test. **p < 0.01; ***p < 0.001.