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. 2019 Jun 14;106(10):1404–1414. doi: 10.1002/bjs.11206

Figure 1.

BJS-11206-FIG-0001-c

Organoid generation and testing Organoids were generated directly from malignant ascites or from solid lesions by first digesting the tissue enzymatically. Three‐dimensional (3D) tumour organoids were grown out by culturing single cells in 3D basement membrane extract (BME) immersed in basal culture medium. For the in vitro HIPEC experiments, organoids were plated on top of a layer of BME, rather than inside the matrix, to maximize drug exposure and simulate the clinical features of microscopic peritoneal metastases (PMs).