Figure 1.

Effects of T‐cell‐derived cytokines on the expression of HLA‐DR, CD80, CD86, and CD58 of neutrophils. (A) The percentage of HLA‐DR⁺, CD80⁺, CD86⁺, and CD58⁺ neutrophils from eight different birch pollen‐allergic individuals after incubation in medium or with 10 ng/mL of recombinant cytokines for 24 h depicted as box plots: boxes show the values within the first and third quartile divided by the median. The whiskers indicate the minimum and maximum of all data. (B) Neutrophils from five different allergic individuals were incubated with pHrodo‐labeled Bos d 5 in the presence of 10 ng (black), 1 ng (dark gray), 0.1 ng (medium gray), and 0.01 ng/mL (light gray) of recombinant cytokines. Squares represent the median values of the percentage of pHrodo⁺ neutrophils, and the dotted line represents the medium control. A single experiment per individual was performed. *p < 0.05, Wilcoxon‐signed ranks test.