Table 1.
Total protein and dsDNA mass balance of the purification of HIV‐1 Gag‐GFP VLPs using a linear gradient elution (Figure 1). S: supernatant; L: loading material; FT: flow‐through; W: wash; P1‐P5: peaks 1‐5
| Sample | Volume [mL] | Total protein [µg/mL] | Total protein % | dsDNA [ng/mL] | dsDNA % |
|---|---|---|---|---|---|
| S | 100 | 310.9 | – | 1387.5 | – |
| L | 100 | 313.1 | 100.0 | 1289.4 | 100.0 |
| FT | 100 | 134.9 | 43.1 | 37.5 | 2.9 |
| W | 15 | <LLOQ | – | <LLOQ | – |
| P1 | 7 | 367.6 | 8.2 | 10.2 | 0.1 |
| P2 | 5 | 275.1 | 4.4 | 310.5 | 1.2 |
| P3 | 6 | <LLOQ | – | 5114.5 | 23.8 |
| P4 | 6 | <LLOQ | – | 3129.6 | 14.6 |
| P5 | 2 | <LLOQ | – | 1108.3 | 1.7 |
| Recovery | 55.7 | 44.3 |
<LLOQ: lower than the lower LOQ