Figure 3.

Tenocyte migration experiments. (a) Migration fold change for dACM conditioned media (CM) and (B) HSAM CM. Mean ± standard deviation reported; n = 24 for each group. **** denotes p < .0001 compared with assay media. (c) Representative images showing tenocyte migration for each group; 4× objective. (d) Scratch assay using assay media, dACM CM, and HSAM CM. Mean ± standard deviation reported; n = 6 for all groups. * denotes p < .05 compared with assay media or dACM CM; ** denotes p < .01 compared with assay media. (e) Representative images showing scratch assay closure for each group; scale bar indicates 100 μm. dACM, dehydrated amnion/chorion membrane; HSAM, hypothermically stored amniotic membrane