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The establishment of miR‐22 overexpression and knockdown models. A‐C, Cells were cultured in the corresponding medium for the indicated times. Efficiency was determined by quantitative PCR and measured as the ratio of miR‐22 relative to the internal reference target U6 gene. *,**,***Significant differences between the tested and the control strains, P < .05, P < .01, and P < .001, respectively. PCR, polymerase chain reaction
