Figure 8.

miR‐22 changes the tumor‐related genes expression in SCLC after γ‐irradiation. A, Samples were cultured in the corresponding medium for the indicated conditions. P value represents the clustering DEGs analysis. Cells were cultured in the corresponding medium for the indicated conditions. mRNA level was determined by quantitative PCR and measured as the ratio of PC and SCUBE1 relative to the internal reference target β‐actin gene. B, Cells were cultured in the corresponding medium for the indicated conditions. mRNA level was determined by quantitative PCR and measured as the ratio of PC, STC1, KLK8, GPM6A, and SCUBE1 relative to the internal reference target β‐actin gene. C, Cells were cultured in the corresponding medium for the indicated conditions. mRNA level was determined by quantitative PCR and measured as the ratio of PC and SCUBE1 relative to the internal reference target β‐actin gene. *, **, ***Significant differences between the tested and the control strains, P < .05, P  < .01, and P  < .001, respectively. DEG, differentially expressed genes; MAPK, mitogen‐activated protein kinases; mRNA, messenger RNA; PCR, polymerase chain reaction; P13K, phosphoinositide 3‐kinases; SCLC, small‐cell lung cancer