Skip to main content
. 2019 Aug 8;145(10):2804–2815. doi: 10.1002/ijc.32594

Figure 4.

Figure 4

VCAM‐1 blockade in vivo does not affect 4T1 tumor growth but inhibits lymphatic invasion. Tumor volumes (a) and endpoint (Day 21) tumor weights (b) of VCAM‐1 deficient 4T1‐1F8 tumors in mice treated with a VCAM‐1 blocking antibody or a corresponding IgG control every other day (n = 10 mice/group). (c) Weight of inguinal (left) and axillary (right) lymph nodes at the endpoint (Day 21; n = 10 mice/group). (d) Left panel: Representative microscopic images showing frequent invasion of tumor cells (stained for pan‐cytokeratin, red) into lymphatic vessels (LYVE‐1, green) in control‐treated tumors but not in tumors treated with the VCAM‐1 blocking antibody. Right panel: Quantification of the frequency of tumor cell‐invaded lymphatic vessels in control and anti‐VCAM‐1 treated tumors (n = 10 control/9 treated tumors; *p < 0.05). [Color figure can be viewed at wileyonlinelibrary.com]