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Zeocin selection of cell lines. (A) After zeocin selection, the transfected DF-1 cells formed a single cell colony within 10 to 15 D. The single cell colony appeared to increase in size over the following 6 to 10 D, and the differences in the size of the cell clones are shown on days 16 (B) and 20 (C). (D) The cells had nearly grown to a monolayer by approximately 21 D in culture. Magnification is ×400 for (A) and 200 × for (B–D).
