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(A) PCR amplification of the env gene from DF-1/B cells. (M) DNA marker 3; (DF-1/B): Genomic DNA extracted from DF-1/B cells; (DF-1): Genomic DNA extracted from DF-1 cells. (B) Verification of the stability of the ALV-B env gene in DF-1/B cells during passages. (M) DNA marker3; Lanes 1–8: ALV-B env gene cell-culture passage levels 15, 25, 30, 35, 40, 45, 50, 60, respectively.
