Figure 5.

Western blot analysis of env protein expression in DF-1/B cells. (DF-1/B): DF-1/B cell lysate; (DF-1): DF-1 cell lysate was used as a negative control. ALV-B env protein in the cell lysates were detected with monoclonal antibodies for ALV-B at a dilution of 1:200. β-actin cell lysates was also detected using actin antibodies for control for equal loading. A IRDye 800-conjugated anti-mouse IgG (1:10,000; Rockland Immunochemicals, Limerick, PA) diluted in PBS was used as the secondary antibody.