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Primers used for amplifying the ALV-B env gene and EGFP fragment in this study.
| Primer1 | Sequence (5′–3′) | Product size (bp) |
|---|---|---|
| B-env-F | CGGGGTACCGCCACCATGGAAGCCGTCATAAAGATGAGGCGA | 1845 |
| B-env-R | AAGGAAAAAAGCGGCCGCCTATACTGCTCTTTCGGGCTGCTTA | |
| EGFP-F | TTGCGGCCGCATGGTGAGCCAAGGGCGAGGA | 720 |
| EGFP-R | GCTCTAGATTACTTGTACAGCTCGTCCA |
F and R represent upstream primer and downstream primer, respectively. Italic for restriction sites, the underlined represents the Kozak sequence.
