Figure 4.

The correlation between GrB or Fas upregulation and Syk, NF‐κB pathways in mouse IFN R^−/− NK cells. Mouse IFN R^−/− NK cells were precultured with the PDTC (500 ng/mL) or herbimycin A (250 ng/mL), prior to incubation with NDV‐HN (500 ng/mL) or a mixture NDV (25 HU/mL) and neutralizing antibodies against HN (1 μL/mL). Precultured or noncultured mouse IFN R^−/− NK cells were stimulated with NDV‐HN (500 ng/mL) for 16 hr. Supernatant was harvested, and concentration of soluble GrB and Fas was determined by ELISA assay. Bars represent the mean ± SD of 3 wells, and similar results were obtained in three independent experiments. *P < 0.05 versus NDV‐HN stimulated.