Figure 5.

Original images (A) and summarized data (B) showing GrB and Fas/FasL proteins expression in mouse IFN R^−/− NK cells. Mouse IFN R^−/− NK cells were stimulated with NDV‐HN (500 ng/mL) for 16 hr. Cells were lysed, and GrB‐ and Fas/FasL‐specific Western blot analysis was performed. As a negative control, unstimulated mouse IFN R^−/− NK cells were used. As a positive control, NDV (25 HU/mL) stimulated mouse IFN R^−/− NK cells were used. β‐actin was used as a loading control. GrB and Fas/FasL expressions in stimulated mouse IFN R^−/− NK cells (stimulated by NDV or NDV‐HN) were obviously higher than unstimulated mouse IFN R^−/− NK cells. Similar results were obtained in three independent experiments, and bars represent the mean ± SD of 3 wells. *P < 0.05versus Unstimulated.