Figure 6.

Original images (A) and summarized data (B) showing the correlation between GrB or Fas/FasL upregulation and Syk, NF‐κB pathways in mouse IFN R^−/− NK cells. Mouse IFN R^−/− NK cells were precultured with PDTC (500 ng/mL) or herbimycin A (250 ng/mL), prior to incubation with NDV‐HN (500 ng/mL) or a mixture NDV (25 HU/mL) and neutralizing antibodies against HN (1 μL/mL). Precultured or noncultured mouse IFN R^−/− NK cells were stimulated with NDV‐HN (500 ng/mL) for 16 hr. Cells were lysed, and GrB‐ and Fas/FasL‐specific Western blot analysis was performed. β‐actin was used as a loading control. GrB and Fas/FasL proteins expressions in precultured mouse IFN R^−/− NK cells were obviously lower than noncultured mouse IFN R^−/− NK cells. Similar results were obtained in three independent experiments, and bars represent the mean ± SD of 3 wells. *P < 0.05 versus NDV‐HN stimulated.