Figure 3.

Dynamic properties of artificially linked $\mathrm{Ub}\genfrac{}{}{0pt}{}{48}{2\text{-}\mathrm{PA}}$ . A) ¹H,¹⁵N hetNOEs of backbone residues comprising the proximal unit of $\mathrm{Ub}\genfrac{}{}{0pt}{}{48}{2\text{-}\mathrm{PA}}$ (blue) compared with monomeric wild‐type Ub (black). The relaxation parameters R ₁ and R ₂ are shown in Figure S7. B) Rate constants (k _HX) for the exchange of amide protons of the proximal unit of $\mathrm{Ub}\genfrac{}{}{0pt}{}{48}{2\text{-}\mathrm{PA}}$ versus protons of the solvent detected in the modified MEXICO experiment. C) Difference in exchange rate constants (Δk _HX) observed for amide protons presented in B) and amide protons comprising wild‐type monomeric Ub.