Table 1.
Immunohistochemistry for Tspan8 in breast cancer primary tumours and in their metastases
| Primary tumour No | Primary tumour | TSPAN8 cytoplasmic | TSPAN8 membrane | Metastasis No | Metastasis | TSPAN8 cytoplasmic | TSPAN8 membrane |
|---|---|---|---|---|---|---|---|
| 1 | Poorly differentiated, invasive ductal | 50% + | – | 1 | Brain | 20% + | 10% + |
| 2 | Poorly differentiated, invasive ductal | 50% + | – | 2 | Brain | – | – |
| 3 | Poorly differentiated, invasive ductal | – | – | 3 | Brain | 10% + | – |
| 4 | Poorly differentiated, invasive ductal | 70% + | – | 4 | Brain | – | – |
| 5 | Poorly differentiated, invasive ductal | – | – | 5 | Brain | – | – |
| 6 | Moderately differentiated, invasive ductal | – | – | 6 | Brain | – | – |
| 7 | Moderately differentiated, invasive ductal | 80% + | – | 7 | Brain | – | 10% + |
| 8 | Moderately differentiated, invasive ductal | – | – | 8 | Brain | – | – |
| 9 | Poorly differentiated, lymphangio‐invasive | 80% + | – | 9 | Brain | 40% + | 10% |
| 10 | Moderately differentiated, invasive ductal | 80% + | single + | 10 | Liver | 80% + | – |
| 11 | Moderately differentiated, NST | 80% ++ | – | 11 | Liver | 80% + | – |
| 12 | Poorly differentiated, invasive ductal | 80%+ | 10%+ | 12 | Liver | 80% + | – |
| 13 | Moderately differentiated, invasive lobular | 90%++ | single + | 13 | Bone | 50% + **/*** | – |
| 14 | Moderately differentiated, invasive lobular | 95%+++ | – | 14 | Bone | 90% + */*** | – |
| 15 | Moderately differentiated, invasive ductal | 80%++ | – | No information | |||
| 16 | Well differentiated, NST | 80%+ | – | No information | |||
| 17 | Well differentiated, invasive ductal | 80%++ | – | No information | |||
| 18 | Moderately differentiated, NST | 80%++ | 5% | No information | |||
| 19 | Moderately differentiated, NST | 80%+ | – | No information | |||
| 20 | Poorly differentiated, invasive ductal | 80%+ | 20%+ | No information | |||
| 21 | Moderately differentiated, invasive lobular | 80%+ | single + | No information | |||
| 22 | Moderately differentiated, invasive lobular | 80%+ | – | No information | |||
| 23 | Moderately differentiated, invasive lobular | 80%++ | – | No information | |||
| 24 | DCIS (G2) | 80%+ | – | ||||
| 25 | DCIS (G2) | 80%+ | 20%+ | ||||
| 26 | DCIS (G2) | 80%+ | 20%+ | ||||
| 27 | DCIS (G2) | 80%+ | single + | ||||
| Moderately differentiated, invasive ductal | 15 | Brain | 50% + | – | |||
| Moderately differentiated, invasive ductal | 16 | Brain | – | – | |||
| Poorly differentiated, invasive ductal | 17 | Brain | – | – | |||
| Moderately differentiated, invasive ductal | 18 | Brain | – | – | |||
| Moderately differentiated, invasive lobular | 20 | Brain | – | – | |||
| Moderately differentiated, invasive lobular | 21 | Brain | – | – | |||
| Moderately differentiated, invasive lobular | 22 | Brain | 50% + | 50% ++ | |||
| Moderately differentiated, invasive lobular | 23 | Brain | – | – | |||
| Moderately differentiated, invasive lobular | 25 | Brain | – | – | |||
| No information | 26 | Brain | 80% +++ | 40% ++ | |||
| No information | 27 | Brain | 80% + | 5% + | |||
| No information | 28 | Brain | 80% + | – | |||
| No information | 29 | Brain | 80% + | – | |||
| No information | 30 | Brain | 80% ++ | 5% + | |||
| No information | 31 | Brain | – | – | |||
| No information | 32 | Brain | 80% + | – | |||
| No information | 33 | Brain | 80% + | – | |||
| No information | 34 | Brain | 80% + | – | |||
| No information | 35 | Brain | 80% + | – | |||
| No information | 36 | Brain | 80% ++ | – | |||
| No information | 37 | Brain | 80% + | 5% + | |||
| Invasive lobular | 38 | Liver | 80% + | – | |||
| Invasive ductal | 39 | Liver | 80% + | – | |||
| No information | 40 | Liver | 80% + | – | |||
| No information | 41 | Liver | 80% + | – | |||
| No information | 42 | Liver | 80% + | 5% + | |||
| No information | 43 | Liver | 80% + | Single + | |||
| Invasive NST | 44 | Lung | 80% + | 10% + | |||
| Invasive, NST | 45 | Lung | 80% ++ | 50% + | |||
| No information | 46 | Lung | 80% + | 50% + | |||
| No information | 47 | Lung | 80% + | – | |||
| No information | 48 | Lung | 80% + | – | |||
| No information | 49 | Lung | 80% + | – | |||
| Moderately differentiated, invasive NST | 49 | Bone | 80% + | Single + | |||
| Moderately differentiated, invasive ductal | 50 | Bone | 80% + | 30% + | |||
| No information | 51 | Bone | 80% + | – | |||
| No information | 52 | Bone | 80% + | – | |||
| No information | 53 | Bone | 80% + | Single + | |||
| No information | 54 | Bone | 80% + | 10% + |
Data for TSPAN8 expression in the primary tumours and their corresponding metastases detected in the same patient are linked horizontally. Only analysed specimens are numbered: thus, there are 27 primary tumours, and 54 metastases were examined.
70% +: 70% epithelium stained at low intensity; −: no TSPAN8 staining was detected.
Intensity was estimated as +, low; ++, moderate; and +++, strong by three pathologists.