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. 2019 May 30;234(12):22921–22934. doi: 10.1002/jcp.28854

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© 2019 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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KPC1 interacts with endogenous Bax and decreases translocation of Bax to mitochondria in H/R H9c2 cells. (a) Two days after treated with Ad‐KPC1 or Ad‐Ctrl, the cells were exposed under H/R conditions. Double fluorescence staining of Bax and Mitotracker were analyzed in H/R cells pretreated with or without Ad‐KPC1. KPC1 decreases Bax translocation to mitochondria from cytoplasm (arrow). (b) Two days after H9c2 cells treated with Ad‐KPC1 or Ad‐Ctrl, the cells were exposed under H/R conditions. The cell lysate were immunoprecipitated with anti‐KPC1 and immunoblotted with anti‐Bax. H/R, hypoxia and reoxygenation [Color figure can be viewed at wileyonlinelibrary.com]