Figure 2.

Staphylococcus lugdunensis tagN encodes a GalNAc‐transferase that produces a macrophage galactose‐type lectin (MGL) ligand. (a) Transfer of SaPI BovI via phage ϕ187 into PS187 wild‐type (WT), GN1 mutant, and GN1 complemented with tagN from S. lugdunensis (pSlug tagN). Values are displayed as transductants per plaque‐forming units (TrU/PFU). In case of GN1 no transductants were obtained. (b) PAGE analysis of wall teichoic acid from Staphylococcus aureus PS187 WT, GN1 mutant, and GN1 complemented with tagN from S. lugdunensis (pSlug tagN). (c) Binding of hMGL to S. aureus PS187 WT, GN1 mutant, and GN1 complemented with either PS187 tagN (ptagN) or pSlug tagN. (d) Interaction of different coagulase‐negative staphylococci species with hMGL. Means of geometric mean fluorescence intensity ± standard error of mean from three independent experiments are shown. ****p < .0001