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. 2019 Jul 8;21(10):e13072. doi: 10.1111/cmi.13072

Figure 3.

Figure 3

Wall teichoic acid‐GalNAc contributes to interaction between human monocyte‐derived dendritic cells (moDCs) and Staphylococcus aureus PS187. (a) Binding of FITC‐labeled S. aureus PS187 WT (green) to immature moDCs (membrane in red, nucleus in blue). Cytospin samples were prepared from cell suspensions incubated with bacteria in 1:50 ratio for 30 min. (b) Binding of FITC‐labeled S. aureus strains to moDCs at different cell‐to‐bacteria ratios after 30 min of incubation. Data are presented as mean ± standard error of mean (SEM) of percentage of FITC‐positive moDCs (n = 3). Significance shown as compared with binding of PS187 WT to moDCs within certain ratio in red for dGN1 mutant and in blue for UAS300 WT. (c) Binding of FITC‐labeled S. aureus PS187 WT and GN1 to moDCs in 1:10 cell‐to‐bacteria ratio after 30 min in the absence or presence of 1 mM EGTA, 50 mM GalNAc or 50 mM glucose (control). Data are presented as a mean ± SEM of percentage of FITC‐positive moDCs (n = 3). *p < .05, **p < .01, ***p < .005