Table 2.
Monoclonal Antihuman Antibodies Used for Flow‐Activated Cell Sorting
| Antibody Marker | Fluorochrome | Clone | Company |
|---|---|---|---|
| CD3 | AF700 | SK7 | BioLegenda |
| CD3 | BV785 | SK7 | BD Biosciencesb |
| CD4 | BV510 | OKT4 | BioLegend |
| CD19 | BV785 | HIB19 | BD Biosciences |
| CD20 | AF700 | 2H7 | BD Biosciences |
| CD21 | BV711 | B‐Ly4 | BD Biosciences |
| CD27 | BV421 | M‐T271 | BD Biosciences |
| CD38 | PE‐Cy7 and PerCP‐Cy5.5 | HIT2 | BioLegend |
| CD49d (VLA‐4) | APC | 9F10 | BD Biosciences |
| CCR4 | PE‐Cy7 | L291H4 | BioLegend |
| CCR6 | PE | G024E3 | BioLegend |
| CXCR3 | BV605 and APC | G025H7 | BioLegend |
| CXCR5 | APC‐R700 | RF8B2 | BD Biosciences |
| IgA | FITC | IS11‐8E10 | Miltenyi Biotechc |
| IgD | PE and PE‐CF594 | IA6‐2 | BD Biosciences |
| IgG | ACP‐H7 | G18‐145 | BD Biosciences |
| IgG1 | PE | HP6001 | Southern Biotechd |
| IgG2 | AF488 | HP6002 | Southern Biotech |
| IgM | BV510 | MHM‐88 | BioLegend |
| T‐bet | PE‐Cy7 | 4B10 | BioLegend |
| Fixable viability dye (FVS) | AF700 | BD Biosciences |
a
BioLegend, London, UK.
b
BD Biosciences, Erembodegem, Belgium.
c
Miltenyi Biotech, Leiden, the Netherlands.
d
Southern Biotech via ITK Diagnostics, Uithoorn, the Netherlands.