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. 2019 Aug 13;58(38):13345–13348. doi: 10.1002/anie.201906737

Figure 1.

Figure 1

a) Synthesis of squaramate‐modified DNA (DNA_CESQ) and its cross‐linking with Sulfo‐Cy5‐NH2, lysine, tri‐ and decapeptide. b) SDS‐PAGE analysis of the incorporation of dCESQTP into DNA using KOD XL polymerase and temp20 1C. P: primerA; (+): natural dNTPs; (−): natural dNTPs without dCTP; CESQ: dCESQTP, dGTP, dTTP, dATP. c) SDS‐PAGE analysis of the conjugation of DNA_CESQ (4.3 μm) with Ac‐Lys (11 mm), tripeptide (11 mm), and decapeptide (11 mm). Conditions: borate buffer (0.5 m, pH 9), 37 °C, 36 h. For the oligonucleotide sequence see Table S1 in the Supporting Information.