Figure 3.

Pathways altered in RUNX2/MYC transgenic thymus. A, Network diagram showing MYC‐regulated pathways altered in RUNX2/MYC thymus, adapted from Ingenuity IPA. Shown are genes with predicted activation (red) or inhibition (blue), with darker colors indicating higher confidence. Red arrow: predicted to lead to activation; blue arrow: predicted to lead to inhibition; gray arrows: no prediction; yellow arrow: the measured findings are inconsistent with the activation state of the downstream molecule. B, Venn diagram showing the interaction between gene sets with either MYC as an upstream regulator (activating) or p53 as an upstream regulator (activating or inhibiting). Bold italics denotes genes with anchored RUNX/MYC binding sites. C, Scatterplot showing the expression of all genes (light blue) and known p53 targets (dark blue)35 in WT and RUNX2/MYC thymus (Control and R2/M, respectively). p53 is highlighted in red. A fold‐change of 1 (no change) is depicted by a solid gray line, whereas fold‐changes of +/− 1.4 are shown as dashed lines. D, Plot showing the fold‐change in expression of p53 modifying enzymes in RUNX2/MYC thymus. Enzymes conferring inhibiting modifications (blue) and activating modifications (green) are shown. The light blue modifiers are those that are significantly altered. Bold indicates the presence of anchored RUNX/MYC binding sites