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. 2019 May 29;49(9):1356–1363. doi: 10.1002/eji.201948143

Figure 1.

Figure 1

(A) Gp33/K9C and Gp34/A8C tetramer‐specific responses by flow cytometry in blood at time‐points 21, 50, 75, 100, and 150 post‐immunization with Ad‐Gp33 compared to naïve mice. Data pooled from three separate experiments up to day 50: n = 13, Day 75 onwards is one experiment (n = 4). Mean ± SEM shown. (B) A8C and K9C tetramer‐specific CD8+ T cells showing CD44, CD62L, and CD127 positivity by flow cytometry at day 21 and 50‐post immunization with Ad‐Gp33. Data shown from one experiment (n = 6), and representative of two independent experiments (n = 12). Mean ± SEM shown. (C) A8C and K9C tetramer‐specific responses by flow cytometry in organs at day 75‐post immunization with Ad‐Gp33, compared to naïve mice. Data shown from one experiment (n = 4), and representative of two independent experiments (n = 8). Mean ± SEM shown. (D) Demonstrates LCMV plaque assays performed on spleens (i) and lymph nodes (inguinal) (ii) at day 4‐post LCMVWE challenge of C57BL/6 mice pre‐immunized (day 50) with Ad‐Gp33 and Ad‐Gp compared to naïve mice. Data are pooled from two separate experiments (n = 6). Mean ± SEM shown.