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. 2019 May 29;49(9):1356–1363. doi: 10.1002/eji.201948143

Figure 2.

Figure 2

(A) Gp34/A8C (i) and Gp33/K9C (ii) tetramer‐specific responses by flow cytometry in blood at time‐points 14, 21, and 50 post‐immunization with Ad‐Gp34 compared to Ad‐Gp33 and naïve mice. Data pooled from two separate experiments: n = 12. Mean ± SEM shown. Unpaired t‐test with Welch's correction: *p ≤ 0.01. (B) A8C and K9C tetramer‐specific CD8+ T cells showing CD44, CD62L, and CD127 positivity by flow cytometry at day 21 and 50‐post immunization with Ad‐Gp34. Data shown from one experiment (n = 6), and representative of two independent experiments (n = 12). Mean ± SEM shown. (C) Gp34/A8C (i) and Gp33/K9C (ii) tetramer‐specific responses by flow cytometry in organs at day 75‐post immunization with Ad‐Gp34, compared to Ad‐Gp33 immunized and naïve mice. Data taken from two pooled experiments (n = 12). Mean ± SEM shown.