Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 29;116(9):2236–2249. doi: 10.1002/bit.27076

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Viscumin expression cassettes. (a) pTRAc‐visFL (visFL), containing the native full‐length viscumin gene including 5′‐untranslated region (5′‐UTR), and coding sequence (ml1p). (b) pTRAc‐CHSLPH‐visAA‐S (visA), encoding the apoplast‐targeted viscumin A chain (rMLA), and pTRAc‐CHSLPH‐visBA‐S (visB), encoding the apoplast‐targeted viscumin B chain (rMLB). (c) Cassettes for viscumin A chain (7rMLAAmpr) and B chain (rMLBAmpr) expression in Escherichia coli . 35SS: Cauliflower mosaic virus double enhanced 35S promoter; UT: Native Viscum album 5′‐UTR; LP: Native V. album signal peptide; LK: Native V. album linker peptide; pA35S: Cauliflower mosaic virus 3′‐UTR/polyA signal. +1: Transcription start; black arrow: open reading frame. CHS: 5′‐UTR of the Petroselinum hortense chalcone synthase gene. LPH: murine heavy chain signal peptide from the Tobacco mosaic virus‐specific mAb24. T7: Enterobacteria phage T7 promoter. rMLA: viscumin A chain coding sequence. rMLB: viscumin B chain coding sequence [Color figure can be viewed at wileyonlinelibrary.com]