Table 1.
Purification and yield of viscumin heterodimer from Nicotiana benthamiana leaves
| Response | Homogenate (SPR) | Filtrate (SPR) | Elution (SPR) | Elution (CE) |
|---|---|---|---|---|
| TSP (g/L) | ~2.80 | ~1.00 | ~0.01 | ~0.01 |
| Viscumin yield (mg/kg) a | 2.22 ± 1.53 (n = 2) | 2.02 ± 0.75 (n = 8) | 1.21 ± 0.34 (n = 8) | 7.2 ± 0.1 (n = 2) |
| Purity (g/g) b | ~0.0002 | ~0.0005 | ~0.0681 | ~0.54 |
| Recovery (g/g) c | 1.00 | ~0.91 | ~0.54 | – d |
Abbreviations: CE, capillary electrophoresis; SPR, surface plasmon resonance; TSP, total soluble protein.
a
Arithmetic mean ± standard deviation.
b
Purity as a fraction of TSP in homogenate, filtrate or elution fractions 1 and 2.
c
Overall viscumin recovery.
d
A CE‐specific recovery cannot be calculated because the method is inadequate to estimate the viscumin concentration in bulk extract.