Fig. 2.

Ectopic Cx expression is associated with a dramatic reorganization of actin and myosin in C6 glioma cells.Left panels, Texas Red-phalloidin staining was combined with immunolabeling of gap junction proteins in control mock transfectants and in C6-Cx43 or C6-Cx32 cells. Top andmiddle panels are stained against Cx43, whereas thelower panel is stained against Cx32 (all fluorescein-labeled). Scale bar, 25 μm. Right panels, Texas Red-Phalloidin staining combined with immunoreactivity against myosin light chain in the same field. Actin and myosin fibers are absent in null transfectants but abundantly co-expressed in both C6-Cx43 and C6-Cx32 cells. Scale bar, 10 μm.