Fig. 8.

Double staining with cytochrome c immunohistochemistry and TUNEL in wild-type mice 2 and 24 hr after transient FCI. Two hours after transient FCI, cytosolic cytochrome c expression was seen as brown in color, whereas there were no TUNEL-positive cells in the ischemic brain (B). A significant amount of TUNEL-positive cells was seen in the entire ischemic MCA territory 24 hr after FCI (C, black areas). At this time point, most of the TUNEL-positive cells showed cytosolic immunoreactivity of cytochrome c (C, brown areas;arrows). There were no TUNEL-positive cells or cytosolic cytochrome c immunoreactivity in the control specimens (A). Scale bar, 0.02 mm.