Table 1.
Retrograde transneuronal labeling of autonomic-related limbic forebrain neurons after injection of PRV into the ventral stomach wall in developing rats
| Group (n) | Diencephalon | Telencephalon | |||||
|---|---|---|---|---|---|---|---|
| DMH | LHA | PVN | CeA | BNST | IC | mPFC | |
| P1–P3.5 (7) | 21 ± 6 | 0 | 232 ± 16 | 1 ± 1 | 0 | 0 | 0 |
| P4–P6.5 (5) | 52 ± 11* | 41 ± 9* | 397 ± 37* | 218 ± 29* | 30 ± 6* | 0 | 0 |
| P8–P10.5 (7) | 62 ± 10* | 135 ± 16** | >5001-a | >5001-a | 139 ± 25** | 78 ± 21** | 95 ± 26** |
Cell counts are mean ± SE. Immunopositive (PRV-infected) neurons were counted bilaterally in multiple 40-μm-thick tissue sections sampled at 160 μm intervals through the indicated forebrain nuclei. Cell counts were corrected according to the method ofAbercrombie (1946). BNST, Bed nucleus of the stria terminalis; CeA, central nucleus of the amygdala; DMH, dorsomedial nucleus of the hypothalamus; IC, insular cortex; LHA, lateral hypothalamic area; mPFC, medial prefrontal cortex; PRV, pseudorabies virus; PVN, paraventricular nucleus of the hypothalamus.
Uncorrected cell count value (see Results).
*Significantly greater than the P1–P3.5 group (p < 0.05).
**Significantly greater than both the P1–P3.5 and P4–P6.5 groups (p < 0.05 for each comparison).