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. 2000 Apr 1;20(7):2567–2574. doi: 10.1523/JNEUROSCI.20-07-02567.2000

Fig. 5.

Fig. 5.

BDNF inhibits apoptosis, which correlates with activation of PI-3 kinase and inhibition of GSK3β in trophic-deprived neurons. Cortical neurons were treated with serum deprivation plus 10 μm MK-801(−S+MK) in the presence or absence of 10 ng/ml BDNF. A, BDNF protected cortical neurons from apoptosis compared with vehicle treated controls (Veh) (**p < 0.0001, ANOVA). Apoptosis was scored 24 hr after the treatment. Addition of 30 μm LY294002 (BDNF+LY) reversed the protective effect of BDNF, suggesting a requirement for PI-3 kinase activity in BDNF protection. B, BDNF induced Akt phosphorylation at Ser-473, indicative of PI-3 kinase activation. Akt phosphorylation (pAkt) was examined by Western analysis at indicated times after BDNF treatment. The blots were reprobed to show that the total level of Akt (Akt) remained constant.C, Quantitation of Akt phosphorylation by densitometric analysis of pAkt Western blots. D, BDNF significantly inhibited GSK3β activity in neurons deprived of trophic support (p < 0.001, ANOVA). Results in allpanels are averages of triplicate determinations in at least two independent experiments. Error bars are SEM.