Fig. 7.

Thrombin upregulates thrombomodulin mRNA expression in cultured astrocytes; quantitative thrombomodulin mRNA expression at different times after thrombin treatment as measured by RT-PCR mimic. Astrocytes were treated with either 2 nmα-thrombin for 9, 24, or 48 hr or vehicle (Control). A, Reverse transcription was made with 3 μg of astrocyte total RNA. PCR was performed with the whole product of the reverse transcription to which various concentrations of mimic were added: 10⁻² amol (1); 5 × 10⁻³ amol (2); 2.5 × 10⁻³ amol (3); 1.25 × 10⁻³ amol (4); 0.625 × 10⁻³ amol (5); and 0.312 × 10⁻³ amol (6). The PCR amplimers were separated by electrophoresis on 1% agarose gels. The gel represents a typical experiment of three independent experiments from three independent astrocyte primary cultures. B, Thrombomodulin mRNA quantification performed by competitive RT-PCR mimic. Results are mean of three independent experiments from three different astrocyte primary cultures. Errors bars are SD.